abstract：BACKGROUND:Recombinant protein production in the yeast Pichia pastoris is usually based on the alcohol oxidase promoters pAOX1 and pAOX2, which are regulated by methanol and strongly repressed by other C-sources, like glycerol and glucose. However, the use of methanol brings several disadvantages, which is why current ...

journal_title：Microbial cell factories

authors： Capone S,Horvat J,Herwig C,Spadiut O

更新日期：2015-07-10 00:00:00

abstract：BACKGROUND:Bacterial biofilms are a preferred mode of growth for many types of microorganisms in their natural environments. The ability of pathogens to integrate within a biofilm is pivotal to their survival. The possibility of biofilm formation in Lactobacillus communities is also important in various industrial and ...

journal_title：Microbial cell factories

authors： Jalilsood T,Baradaran A,Song AA,Foo HL,Mustafa S,Saad WZ,Yusoff K,Rahim RA

更新日期：2015-07-07 00:00:00

abstract：BACKGROUND:Predictable control of gene expression is necessary for the rational design and optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of the most important tools available for controlling gene expression. However, the complex expression patterns of yeast promoters have no...

journal_title：Microbial cell factories

authors： Peng B,Williams TC,Henry M,Nielsen LK,Vickers CE

更新日期：2015-06-26 00:00:00

abstract：BACKGROUND:Bacteriocin-producing Lactic acid bacteria (LAB) have vast applications in human and animal health, as well as in food industry. The structural, immunity, regulatory, export and modification genes are required for effective bacteriocin biosynthesis. Variations in gene sequence, composition and organisation w...

journal_title：Microbial cell factories

authors： Tai HF,Foo HL,Abdul Rahim R,Loh TC,Abdullah MP,Yoshinobu K

更新日期：2015-06-16 00:00:00

abstract：BACKGROUND:Invertase Suc2 was recently identified as a key hydrolase for inulin catabolism in Saccharomyces cerevisiae, whereas the Suc2 activity degrading inulin varies greatly in different S. cerevisiae strains. The molecular mechanism causing such variation remained obscure. The aim of this study is to investigate h...

journal_title：Microbial cell factories

authors： Yang F,Liu ZC,Wang X,Li LL,Yang L,Tang WZ,Yu ZM,Li X

更新日期：2015-04-17 00:00:00

abstract：BACKGROUND:Omega hydroxy fatty acids (ω-OHFAs) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. However, the terminal oxygenation of saturated or unsaturated fatty acids for the synthesis of ω-OHFAs is intricate ...

journal_title：Microbial cell factories

authors： Durairaj P,Malla S,Nadarajan SP,Lee PG,Jung E,Park HH,Kim BG,Yun H

更新日期：2015-04-02 00:00:00

abstract：BACKGROUND:Streptomycetes produce a plethora of natural products including antibiotics and anticancer drugs, as well as many industrial enzymes. Their mycelial life style is a major bottleneck for industrial exploitation and over decades strain improvement programs have selected production strains with better growth pr...

journal_title：Microbial cell factories

authors： van Dissel D,Claessen D,Roth M,van Wezel GP

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:Cellulosic biomass especially agricultural/wood residues can be utilized as feedstock to cost-effectively produce fuels, chemicals and bulk industrial enzymes, which demands xylose utilization from microbial cell factories. While previous works have made significant progress in improving microbial conversion...

journal_title：Microbial cell factories

authors： Li P,Sun H,Chen Z,Li Y,Zhu T

更新日期：2015-02-21 00:00:00

abstract：BACKGROUND:Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signa...

journal_title：Microbial cell factories

authors： Yarimizu T,Nakamura M,Hoshida H,Akada R

更新日期：2015-02-14 00:00:00

abstract：:L-arginine (ARG) is an important amino acid for both medicinal and industrial applications. For almost six decades, the research has been going on for its improved industrial level production using different microorganisms. While the initial approaches involved random mutagenesis for increased tolerance to ARG and con...

journal_title：Microbial cell factories

authors： Shin JH,Lee SY

更新日期：2014-12-03 00:00:00

abstract：BACKGROUND:Oil palm empty fruit bunch (EFB) is a lignocellulosic waste produced in palm oil industry. EFB mainly consists of cellulose, hemicellulose (mainly xylan) and lignin and has a great potential to be reused. Converting EFB to fermentable sugars and value-added chemicals is a much better choice than treating EFB...

journal_title：Microbial cell factories

authors： Li Q,Ng WT,Wu JC

更新日期：2014-11-11 00:00:00

abstract：BACKGROUND:Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be...

journal_title：Microbial cell factories

authors： Hasunuma T,Hori Y,Sakamoto T,Ochiai M,Hatanaka H,Kondo A

更新日期：2014-10-12 00:00:00

abstract：BACKGROUND:Microalgae has been concerned as a potential source of biodiesel in the recent years. However, it is costly to harvest microalgae as it is commonly cultured in water and the cells are too small to harvest. In order to reduce the cost of cultivation and harvesting, it is important to improve the biomass produ...

journal_title：Microbial cell factories

authors： Chen X,Liu T,Wang Q

更新日期：2014-10-01 00:00:00

abstract：:Innovative new genome engineering technologies for manipulating chromosomes have appeared in the last decade. One of these technologies, recombination mediated genetic engineering (recombineering) allows for precision DNA engineering of chromosomes and plasmids in Escherichia coli. Single-stranded DNA recombineering (...

journal_title：Microbial cell factories

authors： van Pijkeren JP,Britton RA

更新日期：2014-08-29 00:00:00

abstract：BACKGROUND:Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on v...

journal_title：Microbial cell factories

authors： Brink HG,Nicol W

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Peptides are increasingly used in industry as highly functional materials. Bacterial production of recombinant peptides has the potential to provide large amounts of renewable and low cost peptides, however, achieving high product titers from Chemically Defined Media (CDM) supplemented with simple sugars rem...

journal_title：Microbial cell factories

authors： Bruschi M,Krömer JO,Steen JA,Nielsen LK

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Under N-limiting conditions, Saccharomyces cerevisiae strains display a substantial variability in their biomass yield from consumed nitrogen -in particular wine yeasts exhibit high growth abilities- that is correlated with their capacity to complete alcoholic fermentation, a trait of interest for fermented ...

journal_title：Microbial cell factories

authors： Crépin L,Sanchez I,Nidelet T,Dequin S,Camarasa C

更新日期：2014-08-19 00:00:00

abstract：BACKGROUND:Ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. These compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. Bio...

journal_title：Microbial cell factories

authors： Nielsen CA,Folly C,Hatsch A,Molt A,Schröder H,O'Connor SE,Naesby M

更新日期：2014-08-12 00:00:00

abstract：:Pseudomonas sp. JHN decolourized and biotransformed 4-chloro-2-nitrophenol (4C2NP) in the presence of additional carbon source. The effect of the various concentrations of the 4C2NP was studied on the decolourization of 4C2NP by Pseudomonas sp. JHN. It was observed that strain JHN decolourized and biotransformed 4C2NP...

journal_title：Microbial cell factories

authors： Arora PK,Bae H

更新日期：2014-08-12 00:00:00

abstract：BACKGROUND:Green microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entiti...

journal_title：Microbial cell factories

authors： Sivakumar G,Jeong K,Lay JO Jr

更新日期：2014-06-03 00:00:00

abstract：:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

journal_title：Microbial cell factories

authors： Cai G,Zheng W,Yang X,Zhang B,Zheng T

更新日期：2014-05-24 00:00:00

abstract：BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor mol...

journal_title：Microbial cell factories

authors： Li Z,Nimtz M,Rinas U

更新日期：2014-03-23 00:00:00

abstract：BACKGROUND:Sabinene, one kind of monoterpene, accumulated limitedly in natural organisms, is being explored as a potential component for the next generation of aircraft fuels. And demand for advanced fuels impels us to develop biosynthetic routes for the production of sabinene from renewable sugar. RESULTS:In this stu...

journal_title：Microbial cell factories

authors： Zhang H,Liu Q,Cao Y,Feng X,Zheng Y,Zou H,Liu H,Yang J,Xian M

更新日期：2014-02-10 00:00:00

abstract：BACKGROUND:Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarb...

journal_title：Microbial cell factories

authors： van der Straat L,Vernooij M,Lammers M,van den Berg W,Schonewille T,Cordewener J,van der Meer I,Koops A,de Graaff LH

更新日期：2014-01-17 00:00:00

abstract：BACKGROUND:Transcriptional engineering has presented a strong ability of phenotypic improvement in microorganisms. However, it could not be directly applied to Actinoplanes teichomyceticus L-27 because of the paucity of endogenous transcription factors in the strain. In this study, exogenous transcription factors were ...

journal_title：Microbial cell factories

authors： Wang H,Yang L,Wu K,Li G

更新日期：2014-01-16 00:00:00

abstract：BACKGROUND:The metabolic capabilities of acetogens to ferment a wide range of sugars, to grow autotrophically on H2/CO2, and more importantly on synthesis gas (H2/CO/CO2) make them very attractive candidates as production hosts for biofuels and biocommodities. Acetogenic metabolism is considered one of the earliest mod...

journal_title：Microbial cell factories

authors： Nagarajan H,Sahin M,Nogales J,Latif H,Lovley DR,Ebrahim A,Zengler K

更新日期：2013-11-25 00:00:00

abstract：BACKGROUND:A number of valuable candidates as tuberculosis vaccine have been reported, some of which have already entered clinical trials. The new vaccines, especially subunit vaccines, need multiple administrations in order to maintain adequate life-long immune memory: this demands for high production levels and degre...

journal_title：Microbial cell factories

authors： Piubelli L,Campa M,Temporini C,Binda E,Mangione F,Amicosante M,Terreni M,Marinelli F,Pollegioni L

更新日期：2013-11-19 00:00:00

abstract：BACKGROUND:Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly tolerated. Therefore, ample efforts are underway to rationally design therapies targeting the HCV non-structural prot...

journal_title：Microbial cell factories

authors： Anwar MI,Iqbal M,Yousef MS,Rahman M

更新日期：2013-11-16 00:00:00

abstract：BACKGROUND:In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration (FDA)-approved for human applications. Widespread use of E. coli in ...

journal_title：Microbial cell factories

authors： Marisch K,Bayer K,Cserjan-Puschmann M,Luchner M,Striedner G

更新日期：2013-06-11 00:00:00

abstract：BACKGROUND:p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmf...

journal_title：Microbial cell factories

authors： Kawai Y,Noda S,Ogino C,Takeshima Y,Okai N,Tanaka T,Kondo A

更新日期：2013-05-07 00:00:00

abstract：BACKGROUND:Extracts and products (roots and/or aerial parts) from Echinacea ssp. represent a profitable market sector for herbal medicines thanks to different functional features. Alkamides and polyacetylenes, phenols like caffeic acid and its derivatives, polysaccharides and glycoproteins are the main bioactive compou...

journal_title：Microbial cell factories

authors： Rizzello CG,Coda R,Macías DS,Pinto D,Marzani B,Filannino P,Giuliani G,Paradiso VM,Di Cagno R,Gobbetti M

更新日期：2013-05-04 00:00:00

abstract：BACKGROUND:In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understood reasons, the produc...

journal_title：Microbial cell factories

authors： Schlegel S,Rujas E,Ytterberg AJ,Zubarev RA,Luirink J,de Gier JW

更新日期：2013-03-12 00:00:00

abstract：BACKGROUND:In past years research has focused on the development of alternative Gram positive bacterial expression systems to produce industrially relevant proteins. Brevibacillus choshinensis is an easy to handle non-sporulating bacterium, lacking extracellular proteases, that has been already shown to provide a high ...

journal_title：Microbial cell factories

authors： D'Urzo N,Martinelli M,Nenci C,Brettoni C,Telford JL,Maione D

更新日期：2013-02-01 00:00:00

abstract：:The biotechnology industry has become a key element in modern societies. Within this industry, the production of recombinant enzymes and biopharmaceutical proteins is of major importance. The global markets for such recombinant proteins are growing rapidly and, accordingly, there is a continuous need for new productio...

journal_title：Microbial cell factories

authors： van Dijl JM,Hecker M

更新日期：2013-01-14 00:00:00

abstract：BACKGROUND:Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, the decline of yeast biological functions during aging may compromise their successful biotechnological use. Life span is controlled by a varie...

journal_title：Microbial cell factories

authors： Orozco H,Matallana E,Aranda A

更新日期：2013-01-02 00:00:00

abstract：BACKGROUND:Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many detai...

journal_title：Microbial cell factories

authors： Claes IJ,Segers ME,Verhoeven TL,Dusselier M,Sels BF,De Keersmaecker SC,Vanderleyden J,Lebeer S

更新日期：2012-12-15 00:00:00

abstract：BACKGROUND:E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E. coli make under fermentative condition is ethanol. However, availability of limited reducing equivalence and generation of c...

journal_title：Microbial cell factories

authors： Munjal N,Mattam AJ,Pramanik D,Srivastava PS,Yazdani SS

更新日期：2012-11-04 00:00:00

abstract：BACKGROUND:High-throughput methods are widely-used for strain screening effectively resulting in binary information regarding high or low productivity. Nevertheless achieving quantitative and scalable parameters for fast bioprocess development is much more challenging, especially for heterologous protein production. He...

journal_title：Microbial cell factories

authors： Rohe P,Venkanna D,Kleine B,Freudl R,Oldiges M

更新日期：2012-10-31 00:00:00

abstract：BACKGROUND:Purine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives i...

journal_title：Microbial cell factories

authors： Peifer S,Barduhn T,Zimmet S,Volmer DA,Heinzle E,Schneider K

更新日期：2012-10-24 00:00:00

abstract：BACKGROUND:The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H:gm. The SefA pro...

journal_title：Microbial cell factories

authors： Jarmander J,Gustavsson M,Do TH,Samuelson P,Larsson G

更新日期：2012-09-03 00:00:00